Journal: Cell Research

Article Title: Fibrous-layer resident Angptl7 + periosteal stem cells sense injury inflammation to orchestrate fracture repair

doi: 10.1038/s41422-025-01202-8

Figure Lengend Snippet: a Schematic diagram of the experimental design for bulk RNA-seq of Angptl7-lineage cells before and after bone fracture. b Volcano plots showing the DEGs in the bulk RNA-seq for Angptl7-lineage cells from the uninjured femurs and fractured femurs at 2 dpf. Red, high expressed at 2 dpf; blue, high expressed in uninjured group. c Heatmap of the DEGs between Angptl7-lineage cells from the fractured femurs at 2 dpf and the uninjured femurs. Red, high expression; blue, low expression. d Construction strategy of Cxcl5-EGFP knock-in mice. e Schematic diagram of the experimental design for the bone fracture of Angptl7-CreER;Rosa26-Ai9;Cxcl5-EGFP mice. f Confocal imaging of the femur sections from Angptl7-CreER;Rosa26-Ai9;Cxcl5-EGFP mice showing the Angptl7-Ai9 + cells and Cxcl5-GFP + cells in the uninjured femur (left) and the injured femur (right) at 2 dpf. Scale bar, 100 μm. g GO analysis of the upregulated DEGs in the Angptl7-lineage cells from the injured femurs at 2 dpf. h GSEA analysis showing the enriched gene signature in Angptl7-lineage cells from the uninjured femurs and fractured femurs at 2 dpf. i Quantification of the concentration of TNF-α in the uninjured femurs and injured femurs at 2 dpf, detected by the electrochemiluminescence assay. Data represent mean ± SD. Two-tailed unpaired t -test was used. n = 4 mice per condition. j–l Schematic diagram of the experimental design ( j ), μCT re-construction ( k ) and quantification ( l ) of the ossified bone formed by Angptl7-lineage cells at 8 weeks after transplantation with vehicle, TNF-α (20 ng/mL) and TNF-α (20 ng/mL) plus JSH23 (10 μM). Data represent mean ± SD. One-way ANOVA followed by Tukey’s test was used. n = 3 mice per condition.

Article Snippet: RNA-seq library preparation were conducted according VAHTS® Universal V6 RNA-seq Library Prep kit manual (Vazyme, NR605-01).

Techniques: RNA Sequencing, Expressing, Knock-In, Imaging, Concentration Assay, Electrochemiluminescence, Two Tailed Test, Transplantation Assay